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ARS Home » Plains Area » Houston, Texas » Children's Nutrition Research Center » Research » Publications at this Location » Publication #77294
Title: DECREASED CHOLESTEROL 7ALPHA-HYDROXYLASE EXPRESSION AND ELEVATED LDL CHOLESTEROL IN GENETICALLY HIGH CHOLESTEROL PIGS.

Author
item Ishimura-Oka, Kazumi
item LI, CHEEMING - BAYLOR COLL OF MEDICINE
item SU, DAI-RONG - BAYLOR COLL OF MEDICINE
item POND, WILSON - USDA/ARS/CNRC

Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 2/28/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary: Interpretive Summary not needed for this 115.

Technical Abstract: Cholesterol 7Alpha-hydroxylase (CYP7) is the central enzyme of cholesterol catabolism and may play a pivotal role on cholesterol homeostasis. We reported the development of the genetically high- and low- cholesterol pig models. At the 7th and 8th generations of the pigs, we used TaqI polymorphism (cc, 2.8 kb; CC, 5.0 kb) for a useful marker to segregate the high- or low-cholesterol trait in the pigs. cc and CC allele were associated with high- and low-cholesterol traits with the mean cholesterol level, 105 and 53 mg/dl, respectively. For further understanding of the molecular mechanism by which plasma cholesterol is regulated in these animals, we studied the plasma FPLC pattern for the cholesterol and gene expression of CYP7, LDL receptor and HMG CoA reductase by Northern blot analysis after 8 week feeding of 0.5 or 0 % cholesterol diet. We found that elevated plasma cholesterol in high cholesterol pigs was accounted for the increased LDL cholesterol. CYP7 expression was remarkably increased in low cholesterol pigs fed with 0 % cholesterol diet. However, 0.5 % cholesterol diet did not significantly increase the CYP7 expression. LDL receptor and HMG CoA reductase expression in liver was suppressed in all the animals. These data suggests that CYP7 may play an important role on cholesterol homeostasis in these pigs.