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Title: REVERSED-PHASE SEPARATIONS OF NITROGENOUS PHOSPHOLIPIDS ON AN OCTADECYL POLYVINYL ALCOHOL PHASE

Author
item Abidi, Sharon
item Mounts, Timothy

Submitted to: Journal of Chromatography
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/25/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary: Phospholipids are important fat-containing substances occurring in plants. Compositions of oilseed varieties have significant bearing on the quality and nutrition values of vegetable oils. Existing analytical methods are inadequate to determine the lipid compositions of the various oilseeds. A new analytical method has been developed for the simultaneous separation and quantitation of the lipids in soybean and canola oils. Components in the lipid mixtures were separated with a polymer column. The new analytical procedure can be used in the routine analysis of the fat-like compounds in vegetable oils including genetically modified oils. Rapid analyses of oil samples can be achieved with good reproducibility. Oilseed growers and food researchers can use the analytical data to obtain useful information on differences in phospholipid levels due to genetic engineering of oilseeds.

Technical Abstract: Molecular species of nitrogenous phospholipids (PLs) phosphatidylcholine (PC), phosphatidylethanolamine (PE), PE derivatives, and sphingomyelin (SP) were separated on an octadecyl polyvinyl alcohol (ODPVA) column by reversed-phase HPLC with UV and evaporative light scattering detection (ELSD). Mobile phases employed variable proportions of acetonitrile, methanol, and water. HPLC-UV of the polar lipids yielded components with peak intensities somewhat different from those obtained by HPLC-ELSD despite discernible similarity in the peak profiles observed in the two detection systems. Incorporation of ammonium hydroxide in mobile phases resulted in a decrease in analyte retention. The mobile phase basicity effect on capacity factors of PE species was significantly greater than that of PC counterparts. The new HPLC-ODPVA-ELSD technique was applied to the analysis of PC and PE molecular species in vegetable oils.