Author
 |
Lynn, Dwight |
|
Submitted to: Society for In Vitro Biology Proceedings
Publication Type: Abstract Only Publication Acceptance Date: 4/21/1997 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: The nucleopolyhedroviruses (NPV, family Baculoviridae) are characterized as having virions of two different phenotypes, those occluded in protein matrices called polyhedra and others which are not occluded (OV and NOV, respectively). In the normal disease process, the OV are responsible for initiating infection in an insect while the NOV are responsible for cell-to-cell transmission of infection. The NOV are also routinely used in cell culture research, primarily because NOV are highly infectious to cells while OV are much less so. In vitro research with OV use alkaline solutions to mimic the in vivo conditions for release of OV from polyhedra. In previous studies, 3,600 to 54,000 polyhedra were required to initiate an infection in cell cultures. This is in spite of the fact that each polyhedra can contain hundreds of OV and in some cases, only one polyhedra is necessary to infect and kill an insect. In my research, I have incorporated trypsin treatment of alkali-liberated OV of gypsy moth NPV. The trypsinization resulted in OV which are much more infectious to a gypsy moth fat body cell line while not improving the OV infectivity to other cell lines. The relative susceptibilities of these cell lines to OV and NOV are also compared. Since there can be productivity problems when repeatedly using NOV to initiate infections in cell cultures, developing a method for using OV will be useful. |
