Author
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King, Jerry |
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Snyder, Janet |
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Submitted to: Book Chapter
Publication Type: Book / Chapter Publication Acceptance Date: 4/10/1997 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Supercritical fluid chromatography (SFC) utilizing pressure, density or temperature programming provides an accelerated analysis method for characterizing many complex lipid mixtures. Nonpolar stationary phases in 50/100 micro m i.d. columns, 5-15 m long have been utilized; lipid species are separated according to their molecular weight and polarity, thus permitting a tentative identification of the lipid solute on the basis of relative retention. SFC has proven to be a valuable diagnostic aid in confirming the effect of supercritical fluid extraction/fractionation processes on lipid-bearing substrates and extracts, for example, the fractionation of wool grease and evening primrose oil with time of extraction. Capillary SFC has also proven advantageous for analyzing minor lipid constituents in food and industrial products, such as cholesterol in foods and tocopherols in vegetable oils at 1-50 mg/100 g of sample levels using a flame ionization detector (FID). This provides a suitable method for determining cholesterol and/or fat soluble vitamins, in neat or extracted samples from analytical SFE, for nutritional-labeling purposes. Capillary SFC has been applied advantageously to monitor transesterification, glycerolysis and hydrolysis reactions performed on common vegetable oils and naturally occurring ester and wax mixtures, allowing the degree of completion of these reactions to be ascertained. Such information can be obtained within 30 min. |
