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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #79648
Title: IDENTIFICATION OF AN EPITOPE ASSOCIATED WITH VIRULENCE ON THE MILLER STRAIN OF PORCINE TRANSMISSIBLE GASTROENTERITIS VIRUS

Author
item Woods, Roger
item Wesley, Ronald

Submitted to: International Virtual Conference on Infectious Diseases of Animals
Publication Type: Abstract Only
Publication Acceptance Date: 4/20/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: A monoclonal antibody (MoAb) directed against an epitope located on or associated with the spike glycoprotein of the virulent Miller strain of porcine transmissible gastroenteritis virus (TGEV) has a higher binding affinity for antigen derived from virulent TGEV than for antigen derived from modified-live Miller virus or commercial vaccine strains of TGEV. Differences in binding activity were observed in radioimmunoassay procedures, indirect fluorescent antibody staining, and virus neutralization. Growth of virulent virus in cell cultures supplemented with the MoAb produced a virus that was less virulent for 3-day-old swine but did not prevent clinical signs. The potential uses of this MoAb in vaccine development and diagnostic assays are discussed.