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Title: POPULATION DYMANICS OF THE CARBOHYDRATE-UTILIZATION-MUTANT NTEROBACTER CLOACAE A-11 AND SEED EXUDATION

Author
item Roberts, Daniel
item YUCEL, IREM - 1275-07-00
item Dery, Pierre
item Buyer, Jeffrey

Submitted to: National American Phytopathology Meetings
Publication Type: Abstract Only
Publication Acceptance Date: 8/30/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Strain A-11 is a mini-Tn5 Km mutant of the bacterial biocontrol agent Enterobacter cloacae strain 501R3. Relative to strain 501R3, strain A-11 has reduced colonization ability in radish and cucumber spermosphere but not in sweet corn or pea spermosphere with assays carried out in natural soil or sterile sand. Strain 501R3 was capable of growth on thirteen of sixteen carbohydrates in aqueous seed extracts detected by gas chromatography. Strain A-11 was capable of wildtype growth on only one of these carbohydrates, fructose. Both strains 501R3 and A-11 had equivalent growth on all amino acids and organic acids tested. Levels of carbohydrate and amino acids in seed exudates, as determined by anthrone and ninhydrin assays, respectively, were highest with sweet corn and pea and lowest with cucumber and radish. The data suggest that the impact on the mutation in strain A-11 is greatest in spermospheres that exude the least carbohydrate and amino acid.