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ARS Home » Research » Publications at this Location » Publication #80708
Title: WATER EXTRACTION OF PLANT TISSUES FOR ANALYSIS BY ION CHROMATOGRAPHY

Author
item Russo, Vincent
item KARMARKAR, S - LACHAT INSTRUMENTS

Submitted to: International Ion Chromatography Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Acid extraction of plant tissues has limitations that can affect ion chromatography (IC) results. Very pure water may be used as an alternative extraction medium to negate masking of ions by acids used in extraction. Tissue from green and red bell pepper fruit, sweet corn internodes, cabbage ewrapper leaves, peach leaves, and cotton and okra leaves and stems were extracted with HPLC-grade water or 5 mM hydrochloric- (HCl), acetic-, or citric-acids. Tissues were ashed, or not, prior to extraction with HCl. Cation and anion IC were performed on extracts of all tissues. Extraction with water, in 95% of occurrences, were equal to values of anions and cations extracted with acids. Extraction of plant tissues with HPLC-grade water is recommended because results were comparable to those extracted with acids, ions were not obscured, and there are fewer waste disposal problems.