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Title: ENHANCED IMMUNOGENICITY AND CROSS-REACTIVITY OF RETRO-INVERSO PEPTIDOMIMETICS OF THE MAJOR ANTIGENIC SITE OF FMDV

Author
item MULLER, S - CNRS, STRASBOURG, FRANCE
item GUICHARD, G - CNRS, STRASBOURG, FRANCE
item BENKIRANE, N - CNRS, STRASBOURG, FRANCE
item Brown, Fred
item VAN REGENMORTEL, MHV - CNRS, STRASBOURG, FRANCE
item BRIAND, J-P - CNRS, STRASBOURG, FRANCE

Submitted to: Journal of Peptide Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/10/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary: Peptides corresponding to the immunodominant loop of foot-and-mouth disease licit levels of neutralizing antibody which are protective in several species. However, these levels are lower than those obtained with the inactivated virus particles which are used in the currently available vaccines. In preliminary studies aimed at enhancing the levels of antibody yproduced by the peptides, retro-inverso peptides composed of D-amino acids have been tested for their ability to elicit antibodies. These studies have shown that levels of antibodies can be obtained which are higher and longer lasting than those obtained with the corresponding L-amino acid peptides.

Technical Abstract: Retro-inverso analogues of peptides corresponding to the major antigenic site 141-159 of VP1 from two foot-and-mouth disease (FMDV) variants have been synthesized and tested for their antigenic and immunogenic properties. Antibodies to the L- and retro-inverso peptides were produced by injecting rabbits with peptides covalently coupled to small unilamellar liposomes containing monophosphoryl lipid A as adjuvant. When compared to the antibody response raised against the L-peptides, the duration of the IgG response that was induced by the retro-inverso peptides was significantly longer and the titer of anti-peptide antisera was much higher. Antibodies to retro-inverso peptides crossed-reacted equally well with the respective parent L-peptides. These results, obtained with a viral sequence which was found previously to represent a good candidate for possible vaccination, show that retro-inverso peptidomimetics could be useful for enhancing the immunogenicity of peptides