Skip to main content
ARS Home » Research » Publications at this Location » Publication #80998
Title: EFFECT OF ORGANIC AMENDMENTS ON SCLEROTIAL GERMINATION OF SCLEROTIUM ROLFSII

Author
item SAUDE, C - OKLAHOMA STATE UNIV
item Melouk, Hassan
item DAMICONE, J - OKLAHOMA STATE UNIV
item PAYTON, M - OKLAHOMA STATE UNIV

Submitted to: American Peanut Research and Education Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 7/11/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Sclerotia from six isolates (JD24, JD52, JD65, JD80, JD121, and MEL) of Sclerotium rolfsii, produced on potato dextrose agar (PDA) and on soil enriched with dried crushed peanut leaves (DCPL), were used to study the effects of organic amendments on germination of sclerotia. Petri plates (9.0 cm), each containing 27.5 g of soil, were amended with 0.25 g of DCPL, ,dried crushed rape greens (DCRG), and rape seed meal (RSM). Filter paper ( cm; Whatman #1) was placed on top of contents of each petri plate. Ten sclerotia were placed on top of the filter paper and wetted to saturation, with either deionized water or aqueous methanol (1%). Germination of sclerotia was assessed at 48, 72, and 97 h after incubation in darkness at 24 C. Germination of sclerotia of all isolates, produced on both PDA and enriched soil (ES), was not affected by the DCPL amendment as compared with wetting agents alone. Germination of sclerotia of isolates JD24, JD52, JD65, JD80, and MEL produced on both PDA and ES was significantly (p value ranged from 0.0004 to 0.059) decreased fro the DCRG amendment as compared with wetting agents alone. In addition, sclerotial germination of isolate JP80 'produced' on PDA was significantly (p=0.009) less for RSM as compared with wetting agents alone. For all isolates except JD121, sclerotia produced on both PDA and ES media and had significantly (p values ranged from 0.0001 to 0.09) higher germination at 96 h than at 72 h of incubation. These data suggest that volatile compounds from the breakdown of rape greens are inhibitory to sclerotial germination of s rolfsii.