Skip to main content
ARS Home » Research » Publications at this Location » Publication #81037
Title: GENOME DIVERSITY AND CITRUS PARASITISM AMONG BURROWING NEMATODES FROM HAWAII, CENTRAL AMERICA, AND FLORIDA

Author
item Kaplan, David
item OPPERMAN, CHARLES - NORTH CAROLINA STATE UNIV

Submitted to: Journal of Nematology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/18/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary: Burrowing nematodes from Central America, Dominican Republic, Florida, Guadeloupe, Hawaii, and Puerto Rico were characterized for their ability to parasitize citrus, but citrus parasites were only found in Florida. Biochemical and molecular anlayses indicated that burrowing nematodes distributed world-wide on bananas appear to be highly similar to the citrus parasites in Florida. Findings did not substantiate previous claim that R. citrophilus was present in Hawaii. Citrus parasitism in burrowing nematodes present in Florida appears to be associated with limited changes in the burrowing nematode genome.

Technical Abstract: Burrowing nematodes from Central America, Dominican Republic, Florida, Guadeloupe, Hawaii, and Puerto Rico were characterized for their ability to parasitize citrus, but citrus parasites were only found in Florida. Sequence tag sites originally amplified from a citrus-parasitic burrowing nematode were polymorphic among 38 burrowing nematode isolates and were not correlated with citrus parasitism, nematode isolate collection site, or amplification of a 2.4kb sequence tag site (DK#1). Results of a RAPD analysis and characterization of the isozymes phosphoglucose isomerase, lactate and malate dehydrogenase indicate that burrowing nematode isolates were highly similar. Findings did not substantiate the presence of R. citruophilus in Hawaii and citrus parasitism in Florida appears to be associated with limited changes in the burrowing nematode genome.