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Title: EFFECT OF RBST ON NEUTROPHIL DIAPEDESIS (ND) DURING EXPERIMENTALLY INDUCED S. UBERIS MASTITIS

Author
item SMITS, E
item BURVENICH, C
item Guidry, Albert
item HOEBEN, D
item EPPAND, P

Submitted to: International Virtual Conference on Infectious Diseases of Animals
Publication Type: Abstract Only
Publication Acceptance Date: 9/1/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Neutrophils are the major defense against bacterial infection in the bovine mammary gland. Neutrophils diapedese from blood through secretory epithelial cells into the lumen of the gland in response to inflammatory stimuli. The influence rBST on ND was investigated on 10 cows with experimentally induced Streptococcus uberis mastitis. Cows were divided into 2 groups: a control group and a rBST group. After a pre-inoculation control period of 1 wk, both groups were infected IM on d0 with 500 cfu S. uberis. In the rBST group, 500 mg rBST (Posilac) was subcutaneously administered on d7 prior to and after infection. In the control group, the vehicle only was administered. Blood samples were taken during the pre- inoculation period and from the first to the 28th day after infection. ND was studied using a cell culture model that consisted of a confluent monolayer of bovine secretory mammary epithelial cells on collagen-coated inserts and bovine C5a as the chemoattractant. ND was measured after 5h and occurred from the basal to the apical side of the epithelial cells. One day after infection, ND ws severely reduced in both groups. This decrease in ND continued for several days but was restored 21d after infection. Treatment with rBST had no significant effect on ND. The results showed that during experimental S. uberis mastitis, ND was significantly decreased in both groups and that there was no significant effect of rBST on ND. This study was supported by the Belgian Ministry of Agriculture (grant D1/2-5741A)