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Title: CONSTRUCTION OF A RECOMBINANT CVI988 STRAIN EXPRESSING VIRULENT EPITOPE ON 38 KD PHOSPHORYLATED PROTEIN OF MAREK'S DISEASE VIRUS

Author
item CUI, ZHIZHONG - YANGZHOU UNIVERSITY CHINA
item Lee, Lucy

Submitted to: World Veterinary Poultry Association
Publication Type: Abstract Only
Publication Acceptance Date: 8/18/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: To study the biological roles of a 38 kd phosphorylated protein (pp38) in the pathogenicity of Marek's disease virus (MDV), a recombinant MDV strain CVI/rpp38 was constructed by transfection of vaccine strain CVI988/Rispens infected chicken fibroblast cells (CEF) with plasmid pHA25 DNA (containing the whole pp38 gene from virulent MDV strain GA). Lipofectamine solution was used for transfection. The recombinant CVI/rpp38 was screened and cloned (for both cell-associated and cell-free virus clones) using fluorescence antibody test (FA) with monoclonal antibody (Mab) H19 recognizing all tested serotype I MDV except vaccine strain CVI988. When inoculated into 3-day-old chicks of Line 15X7, the recombinant CVI/rpp38 induced significantly lower FA titers to MDV-CEF than the native cloned CVI988/rispens strain 3 weeks after inoculation (1:65 +/ 33 vs 1:378 +/ 158, p<0.001). The fact that chicks inoculated with the recombinant CVI/rpp38 expressing pp38 of virulent MDV strains gave lower antibody titers to MDV was coninsitant with our previous report that the recombinant pp38 expressed in insect cells demonstrated some immunodepressive effects. The recombinant CVI/rpp38 will potentially be a useful tool to further understand pp38's biological function in reduced immune responses and oncogenesis due to MDV infection.