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Title: EFFECT OF RBST ON NEUTROPHIL DIAPEDESIS (ND) DURING EXPERIMENTALLY INDUCED S. UBERIS MASTITIS

Author
item SMITS, ELKE - UNIV OF GENT, BELGIUM
item BURVENICH, CHRISTIAN - UNIV OF GENT, BELGIUM
item Guidry, Albert
item HOEBEN, DAGMAR - UNIV OF GENT, BELGIUM
item EPPARD, PHILIP - UNIV OF GENT, BELGIUM

Submitted to: International Virtual Conference on Infectious Diseases of Animals
Publication Type: Abstract Only
Publication Acceptance Date: 7/1/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Neutrophils are the major defense against bacterial infection in the bovine mammary gland. Neutrophils diapedese (ND) from blood through secretory epithelial cells into the lumen of the gland in response to inflammatory stimuli. The influence rBST on ND was investigated on 10 cows experimentally infected with S.uberis - 5 nontreated and 5 treated with rBST. The rBST group received 500 mg rBST (Posilac R) subcutaneously on 7 days prior to and after infection. Blood samples were taken during the pre-inoculation period and from the 1st to the 28th day after infection. A cell culture model that consists of a confluent monolayer of bovine secretory mammary epithelial cells on collagen coated inserts was used for studying ND. ND occurred from the basal to the apical side of the epithelial cell monolayers. Bovine C5a was used as chemoattractant. One day after the inoculation of S.uberis, ND was severely reduced in both groups. The threefold decrease was continued for several days and restored 3 wks after infection. A treatment with rBST had no significant effect on ND across bovine mammary epithelial cells with bovine C5a as chemoattractants for a 5h incubation period. The results showed that during experimental S.uberis mastitis, ND is significantly decreased in both groups and that there was no significant influence of rBST on ND.