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Title: ANNUAL REPORT TO NE112 - RESISTANCE TO MASTITIS IN DAIRY CATTLE

Author
item Guidry, Albert
item Paape, Max

Submitted to: NE112 Meeting
Publication Type: Experiment Station
Publication Acceptance Date: 10/20/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: This report is a summary of the major accomplishments of Drs. M.J. Paape and A.J. Guidry for 1996. Specific antibodies in dry secretions were shown to be ineffective in supporting phagocytosis of S. aureus by PMN due to the viscosity of the secretions. Produced two monoclonals (11G10 and 2G8) that inhibited chemotactic activity of bovine neutrophils, up-regulated the amplitude of native chemiluminescence (CL), and shortened the time to maximal CL induced by serum-opsonized zymosan. Showed that IgGl alone or after crosslinking with second antibody, did not induce [Ca2+] I influx and protein tyrosine phosphorylation responses. However IgG2 alone, and crosslinked IgG2 and aggregated 1gG, did cause both Ca2+ influx and protein tyrosine phosphorylation. Seratyped S. aurens isolated from throughout the US, using type 5 and 8 typing sera. Showed that the majority of isolates that typed were type 8, but more importantly 59% were not typeable with either type 5 or 8. This data suggests that current vaccines that incorporate type 5 or 8 have limited potential for success in the US. Both fibronectin and callagen were shown to block binding of S. aureus to mammary epithelial cells. These data wil help dairy researchers produce effective vaccines against Staphylococcus aureus induced infections of the mammary gland (mastitis).