Author
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Abbott Dr, Thomas |
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Wolf, Walter |
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Submitted to: Association for the Advancement of Industrial Crops Conference
Publication Type: Abstract Only Publication Acceptance Date: 6/1/1997 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Simmondsin and its analogues have been extracted from meal with various solvents and their concentrations measured by a number of chromatographic procedures. The ability to completely extract simmondsins depends on the substrate to some degree, but the range of polarities of the simmondsin analogues makes solvent selection difficult. Water, acetone, methanol, 90/10 acetonitrile/water and other solvents have been recommended as the extraction solvent. Analytical high pressure liquid chromatography procedures have varied as well, leading to inconsistent results between laboratories. This presentation will discuss the limitations of some methods for extracting jojoba meal or water-soluble concentrate and measuring simmondsin analogue concentrations. Jojoba proteins have been separated by gel electrophoresis to show a predominant protein at about 25 kilodaltons (kDa) molecular size and a smaller amount of protein at about 47 kDa. By scanning the gels with a densitometer and analyzing the densitometer scans in GRAMS 386, it was shown that the 25 kDa protein consists of at least 3 proteins which vary significantly in concentration. The function of these proteins is not known, but this method could be used to select plants high in one of the proteins to facilitate elucidation of their function in the plant and their functional properties for potential industrial use. |
