THE PRODUCTION OF CYCLODEXTRINS USING CGTASE FROM BACILLUS MACERANS

Authors: Rendleman Jr, Jacob

Submitted to: Methods in Molecular Biology
Publication Type: Book / Chapter
Publication Acceptance Date: 8/5/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Detailed procedures are given for converting starch into alpha-,
beta-, and gamma-cyclodextrin (CD) by means of a combination of
Bacillus macerans cyclodextrin glucanotransferase (CGTase; EC 2.4.1.19)
and a complexant that not only enhances yields but also favors the
production of only one of the three CDs. Pullulanase (EC 3.2.1.41;
from Enterobacter aerogenes), a debranching enzyme, is employed in many
of the reactions in order to produce yields higher than those
attainable in its absence. Decan-1-ol is used as complexant to enhance
alpha-CD production. For beta-CD production, beta-specific complexants
of several different types (cyclohexane, cyclooctane, cyclododecene,
isooctane, and t-butanol) are employed. Cyclododecanone and
cyclotridecanone serve to enhance gamma-CD yields. The methodologies
provide information on conducting the conversion reactions,
inactivating enzymes, removing complexants by azeotropic distillation,
isolating and purifying the products, and analyzing CDs by HPLC.