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ARS Home » Research » Publications at this Location » Publication #83523
Title: SELECTIVE EXTRACTION OF PHOSPHOLIPIDS FROM SOYBEANS WITH SUPERCRITICAL CARBON DIOXIDE AND ETHANOL

Author
item MONTANARI, LUIGI - UNIV PERUGIA PERUGIA ITLY
item FANTOZZI, PAOLO - UNIV PERUGIA PERUGIA ITLY
item Snyder, Janet
item KING, JERRY

Submitted to: Proceedings Of The Italian Conference On Supercritical Fluids
Publication Type: Proceedings
Publication Acceptance Date: 9/7/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Supercritical carbon dioxide (SC-CO2) is very effective in removing oils from a variety of seed matrices, devoid of any appreciable amount of phospholipids (PLs) content. However, the limited solubility of PLs in SC-CO2 leaves behind a potentially valuable by-product in the spent seed matrix (defatted soybeans) that could be recovered to economic advantage. Any PL extraction process from defatted soybeans must maintain the structure and the quality of the PLs and must be compatible with the end use of the seed protein meal as an animal feed or for use in human consumption. For this reason, supercritical fluid extraction is particularly appropriate, since the chosen extracting agent is both environmentally acceptable and non-toxic to food consumers. Ethanol, which is permitted by food safety regulations, enhances the PL solubility on a thermodynamic basis. PL extraction is possible when ethanol is used as a cosolvent in SC CO2. An initial SC-CO2 extraction of soybean flakes was performed at 68.2 MPa and 80 deg C to extract the oil, leaving the PLs in the defatted soybean flakes (DSF). A second step was performed on the DSF using a Xeth= 0.10, varying the pressure from 16.6 to 68.2 MPa and the temperature from 60 to 80 deg C. For all SC-CO2/ethanol extractions, a fraction rich in PLs was obtained. Quantitative and qualitative analysis of PLs in soybean seeds, DSF, and different extracted phospholipid fractions were carried out by HPLC-ELSD analysis.