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ARS Home » Pacific West Area » Aberdeen, Idaho » Small Grains and Potato Germplasm Research » Research » Publications at this Location » Publication #83634
Title: PERFORMANCE OF MALTING BARLEYS DERIVED FROM ANTHER CULTURE

Author
item Hang, An
item SATTERFIELD, KATHY - UNIVERSITY OF IDAHO
item Burton, Charlotte

Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: 3/15/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Seeds of 3 lines of doubled-haploid plants derived from anther culture of 'Andre', 9 lines from 'Crystal', 60 lines from 'Harrington', 7 lines from 'Klages', 12 lines from 'Moravian III', and 10 lines from 'Morex' were planted together with their original cultivars at two locations, one under irrigation at Aberdeen and one on dryland at Tetonia, Idaho, in four replications at each location. The following characteristics were recorded: heading date, plant height, 100-seed weight, and seed yield. To detect the RAPD marker variation from doubled- haploid plants and cultivars, seeds of anther culture derived lines and cultivars were planted in 4-inch pots in the greenhouse, ten seeds per line or cultivar per pot. When the seedlings reached the 3-4 leaf stage, one leaf from each plant was cut and bulked for DNA extraction. PCR technique was used with 22 primers. The results indicated that the majority of derived lines from anther culture tended to be later and taller than the original cultivars. However, one derived line from Moravian III was shorter than the cultivar. Three derived lines from Andre had higher seed weight and seed yield at two locations. Similar results were observed from one derived line from Harrington. In PCR-RAPD variation studies, DNA polymorphism was found in Moravian III derived lines with three primers. Polymorphism also was found in Harrington derived lines with one primer. These observations indicated that DNA had been modified during the process of culture and that this could lead to enhancement of some characteristics in certain cultivars.