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ARS Home » Research » Publications at this Location » Publication #84024
Title: MAPPING CHICKEN GENES USING PREFERENTIAL AMPLIFICATION OF SPECIFIC ALLELES

Author
item Smith, Eugene
item Cheng, Hans

Submitted to: Microbial and Comparative Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/1/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary: Fourteen additional genes have been placed on the East Lansing chicken gene map using a reference population that was based on mating Jungle Fowl sires with White Leghorn dams. Because the Jungle Fowl is the progenitor of domesticated chickens, the probability of locating mutated forms of known genes is enhanced. Locating known chicken genes will provide a framework of anchor loci for comparing regions on chromosomes that have remained linked throughout evolution with those of other species. Ultimately, comparative gene maps will provide additional genetic markers for pinpointing genes of commercial importance such as disease resistance and productivity in chickens.

Technical Abstract: To map the chicken genome, an international reference population, was developed at East Lansing using an F2 backcross between inbred Jungle Fowl (JF) and inbred White Leghorns (WL). To augment the number of Type 1 genes on the East Lansing (E) map, segregation of the JF-specific allele was followed using preferential amplification of specific alleles (PASA) in npolymerase chain reactions (PCR). Among 15 functional genes that were added to the E map, agrin and mannose -6-phosphate receptor genes were found to occur in conserved syntenic groups. Using this PCR-based approach, six conserved groups spanning more than 243 centiMorgans (cM) in the chicken were syntenic with human and mouse.