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Title: CONSERVATION OF LINKAGE OF RAPD AND SCAR MARKERS TO THE RPF1 RESISTANCE GENE FOR PHYTOPHTHORA FRAGARIAE IN STRAWBERRY

Author
item HAYMES, K - CPRO/DLO, THE NETHERLANDS
item VAN DE WEG, W - CPRO/DLO, THE NETHERLANDS
item MEULENBROEK, E - CPRO/DLO, THE NETHERLANDS
item Maas, John
item VOSMAN, B - CPRO/DLO, THE NETHERLANDS
item DEN NIJS, A - CPRO/DLO, THE NETHERLANDS

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 3/20/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary: The strawberry disease red stele root rot, caused by the fungus Phytophthora fragariae var. fragariae, is economically important to strawberry production worldwide in the northern hemisphere. The presence of this pathogen limits strawberry production in many areas of the United States and Canada. However, the USDA and Agriculture Canada each have breeding programs to produce varieties resistant to red stele, as does The Netherlands. Traditional breeding methods may be improved by identifying molecular markers in the DNA of prospective parents that are closely tied to genes for resistance. Several such markers were identified and shown to be inherited in the same manner as the resistance genes, thus providing an aid in selecting parents for breeding resistant varieties and identifying the specific genes that resistant types may possess. By this process, pyramiding of resistance genes may be possible in the future and the time required for traditional breeding processes and evaluation of resistant seedlings will be shortened, making more economical use of resources while producing disease resistant strawberry varieties for the commercial grower.

Technical Abstract: European and North American strawberry (Fragaria sp.) genotypes were evaluated for the presence of random amplified polymorphic DNA (RAPD) and sequence characterized amplified region (SCAR) markers previously found to be linked to the Rpf1 gene. This gene confers resistance to red stele root rot disease, caused by Phytophthora fragariae var. fragariae. The markers were generally present in resistant genotypes and absent in susceptible genotypes. The Rpf1 region thus showed to be highly conserved. Therefore, these RAPD and SCAR-PCR markers can be used in pedigree-analysis studies, cultivar identification, and in breeding programs for the selection of red stele (Rpf1) resistant genotypes.