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Title: OVERVIEW OF THE SECOND INTERNATIONAL WORKSHOP TO DEFINE SWINE CLUSTER OF DIFFERENTIATION (CD) ANTIGENS

Author
item SAALMUELLER, A - FED RES CTR VIRUS DIS
item PAULY, T - FED RES CTR VIRUS DIS
item Lunney, Joan
item Boyd, Patricia
item AASTED, B - ROYAL VET UNIV
item SACHS, D - MASS GEN HOSPITAL
item ARN, S - MASS GEN HOSPITAL
item BIANCHI, A - CENTRAL VET INST
item BINNS, R - AFRC INST ANIM PHYSIOL
item LICENCE, S - AFRC INST ANIM PHYSIOL

Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/12/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary: Scientists worldwide participated in the Second International Swine CD Workshop to verify the reactivity of monoclonal antibodies (mAbs) reactive with pig immune cells. Such international standardization is required so that independently derived reagents can be used in different laboratories for the same types of immune analyses. Important new mAb reagents are needed by the research community to aid in studies of swine disease and vaccine responses. After 2 years of testing the reactivity of the panel of 195 mAb in laboratories internationally, the specificity of a large number of these mAb was confirmed. A total of 23 new mAb were determined to be reactive with definced pig T cell CD antigens, including many pan-T (anti-CD3) mAbs, a CD subset for which previously no mAb had been identified. Unfortunately, for many of the new T cell reactive mAb specific tests indicated that they recognized the same area (epitope) of the CD antigen as previously characterized mAb. Only for wCD8 were mAb tha reacted with unique determinants of the wCD8 molecule defined. Several mAb reactive with B cell and macrophage markers were identified as well as CD45 memory cell markers. Firm characterization of mAb which recognize unique swine immune cell determinants will enable scientists to clearly differentiate unique subsets of swine T cells and to assess fully the functions of these important lymphoid cell subpopulations as they define swine immune cell interactions and thus characterize cells and factors which regulate disease and vaccine responses.

Technical Abstract: The aim of the Second International Swine CD workshop, supported by the Veterinary Immunology Committee (VIC) of the International Union of Immunological Societies (IUIS), was to standardize the assignment of monoclonal antibodies (mAb) reactive with porcine leukocyte differentiation antigens and to define new antibody clusters. At the summary meeting of the workshop in July, 1995, revisions in the existing nomenclature for Swine CD were approved, so that the rules are now in accord with those for human and ruminant CD. Swine CD numbers will not be given to clusters of mAb to swine orthologues of human CD molecules when homology is proven by 1) suitable tissue distribution and lymphoid cell subset expression; 2) appropriate molecular mass of the antigen recognized by the mAbs, and 3) reactivity of mAbs with the cloned swine gene products, or cross-reactivity of the mAb on the human gene products. In some cases this reactivity would not be fully proven, mainly due to the lack of cloned gen producsts; for these CD antigens, the respective clusters will be assigned by the previs "w" which will lead to "wCD" antigens. As a results of the Second International Swine CD Workshop the assignment of 16 mAb to existing CD groups (CD2a, CD4a, DC5a, wCD6, wCD8, CD14, wCD21, wCD25) was confirmed, and 2 mAb to existing SWC. More importantly for the work on the porcine immune system, was the definition of 5 new swine CD antigens, namely CD3 (recognized by 6 new mAb and 3 epitopes), CD16 (1 new mAb), wCD29, (2 mAb), CD45RA (3 mAb) and CD45RC (1 new mAb). Finally, the demarcation of two new SWC molecules in swine, SWC8 (2 mAb) and SWC9 (2 mAb) was confirmed.