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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Publications at this Location » Publication #85172
Title: A MATRIX ATTACHMENT REGION IS FOUND UPSTREAM OF AN ALPHA-AMYLASE GENE IN WHEAT

Author
item CHRISTOFFERS, MICHAEL - UNIVER OF MISSOURI
item Gustafson, J

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/10/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Chromatin organization is thought to involve loop domains formed by attachment of DNA sequences termed matrix attachment regions (MARs) to the proteinaceous nuclear matrix. Wheat (Triticum aestivum L.) alpha-amylase (alpha-Amy3) upstream flanking regions were analyzed for potential MARs by performing binding experiments with nuclear matrices. We have used the lithium diiodosalicylate (LIS) extraction method to isolate nuclear matrices from wheat germ nuclei. Restriction enzyme digests of an alpha- Amy3 genomic clone were incubated with nuclear matrix preparations (exogenous assay) using the endogenous wheat germ DNA as competitor. Matrix-bound DNA was pelleted and compared with unbound supernatant DNA by Southern hybridization. An XbaI fragment of about 1.5 kb was found to preferentially bind nuclear matrices, indicating the presence of a MAR. Sequence analysis revealed a region approximately 0.6 to 1.3 kb upstream of the transcription start site with a high AT-content (greater than 70%) characteristic of MARs. Numerous sites with homology to proposed MAR consensus sequences were found within this region.