Author
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CREGAN, PERRY |
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Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only Publication Acceptance Date: 9/6/1997 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Simple sequence repeat (SSR's) are a valuable source of genetic markers in plants, however, the use of primer sets developed in one species to amplify loci in related species is poorly studied. The use of primer sets in heterologous species would provide a savings in terms of marker development and might offer the opportunity to conduct studies of comparative genome structure. It was our intent to provide initial data on the use of a diverse set of cultivated soybean-derived SSR markers on other species in the genus Glycine. The study involved a total of 19 Glycine accessions including cultivated and wild soybean (the putative ancestor of cultivated soybean) as well as the 17 perennial species. PCR amplification of genomic DNA of each of the 19 genotypes was attempted using primer sets to 72 SSR loci derived from cultivated soybean. The SSR core motifs included AT, CT, ATT, and CTT. Eleven of the loci were derived from GenBank sequences while the remainder were derived from genomic libraries. Fifteen loci gave products only in cultivated and wild soybean while 19 yielded amplification products in all 19 genotypes. A cluster analysis based upon the presence or absence of a PCR amplification product with each genotype/primer set combination gave results that were similar but clearly not identical to other reports that have attempted to define relationships between Glycine species. |
