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ARS Home » Research » Publications at this Location » Publication #85523
Title: SIMPLE SEQUENCE REPEAT (SSR) MARKER DEVELOPMENT IN THE WHEAT GENOME. ISOLATION, ANALYSIS AND CHARACTERIZATION

Author
item Tamulonis, John
item Cregan, Perry

Submitted to: American Society of Agronomy Meetings
Publication Type: Abstract Only
Publication Acceptance Date: 9/6/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Wheat (Triticum aestivum L. em. Thell. and T. turgidum) is the world's leading cereal grain and most important food crop. Two genomic DNA library screenings revealed that 2.43% of the wheat DNA fragments contained an SSR indicating that microsatellite sequences are abundant in the wheat genome. A total of 232 clones were sequenced and 95% contained SSRs. Primer pairs were synthesized from 47% of the clones and twenty-two genome specific SSR loci were identified. The 22 loci were tested on three putative progenitor species and eight cultivated wheat genotypes. The number of alleles per locus was 4.5 with an average polymorphism information content of 0.65. Two SSR loci were mapped in the M6 x Opata RIL population. One SSR locus linked to marker Xbcd926 on chromosome 5A and the other was linked to marker Xcdo959 on chromosome 4A. SSR markers have wide applicability in genetic mapping and marker facilitated plant improvement.