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Title: DNA FINGERPRINTING OF STRAWBERRY (FRAGRIA X ANANASSA) CULTIVARS USING RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS

Author
item DEGANI, CHEMDA - VOLCANI CENTER, ISRAEL
item Rowland, Lisa
item LEVI, AMNON - USDA, ARS, FRUIT LAB
item HORTYNSKI, JERZY - UNIV OF AGRICULT, POLAND
item Galletta, Gene

Submitted to: Journal of Euphytica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/27/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary: Because of the need to maintain cultivar purity in the strawberry industry, DNA fingerprinting of 41 of the major strawberry cultivars grown in the United States and Canada was performed using DNA markers known as RAPDs (random amplified polymorphic DNA). A set of seven RAPD primers produced 10 DNA markes which were sufficient to distinguish among all the 41 tested cultivars. A DNA fingerprinting table was constructed based on these results. In addition, RAPD marker data was used to estimate the genetic relatedness of cultivars and this information was compared to known parentage information for the cultivars. Our results demonstrate that RAPD markers can easily distinguish different strawberry cultivars, even closely related ones, some of which were full sibs or parents of each other. Consequently, DNA fingerprinting using RAPD markers should be useful in maintaining cultivar purity in strawberry germplasm collections.

Technical Abstract: Forty-one of the major strawberry (Fragaria x ananassa Duch.) cultivars grown in the United States and Canada were examined for RAPD (randomly amplified polymorphic DNA) marker polymorphisms using 10mer primers (>50% GC content). A set of 10 primers produced 15 polymorphic fragments ranging in size between 450 and 1200 bp, which were more than sufficient to distinguish among all tested cultivars. Ten of the markers derived from seven primers were absolutely required for distinguishing the cultivars. A DNA fingerprinting table was constructed based on these results. In addition, similarity coefficients were calculated based on RAPD marker data and a dendogram was constructed using the unweighted pair group method of arithmetic averages (UPGMA). These results were compared with known pedigree data for the cultivars. Our results demonstrate that RAPD markers can be used effectively for strawberry cultivar identification.