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Title: EXACERBATION OF LACTATE ACCUMULATION IN AN IN VITRO RUMINAL FERMENTATION MODEL BY MONILIFORMIN (A MYCOTOXIN)

Author
item WU, W - U OF WI, MADISON
item SCHAEFFER, D - U OF WI, MADISON
item COMBS, D - U OF WI, MADISON
item Vesonder, Ronald

Submitted to: Conference on Rumen Function
Publication Type: Abstract Only
Publication Acceptance Date: 11/13/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: In vitro batch fermentation consisting of equal volume of ruminal fluid (as inoculum taken from a fistulated cow fed a diet with 66.6% alfalfa hay and 33.4% concentrate) and McDougall's buffer (pH adjusted to 6.0 with HCl) and a substrate (60 mM of glucose or 250 mg of alfalfa hay powder) was carried out at 39 C for 24 h to observe the effects of moniliformin (MN) on concentration changes of lactate, glucose, and volatile fatty acids (VFA). Only glucose formed an acidotic model. Compared with control, significantly 30% more lactate accumulated in the fermentation containing 25 uM (micromoles/liter) of MN by 3 h of incubation. Same effect was observed by 1.5 h with 50 uM or more on MN. More lactate (>30 to 120% of control) was observed towards 24 h of incubation. Transitional higher lactate (>30% of control) was observed with 4.5 and 6 h samples from fermentation containing 5 uM of MN. MN did not affect glucose and VFA metabolism. Addition of 100 uM of thiamin did not negate the effect of 50 uM of MN on lactate accumulation, suggesting that the mode of action did not relate to thiamin. MN (100 uM) did not affect the alfalfa fermentation model. Since MN-producing molds have been recovered from high moisture corn associated with chronic acidosis, we postulated that MN can exacerbate grain overfed acidosis.