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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Ruminant Diseases and Immunology Research » Research » Publications at this Location » Publication #86600
Title: CLONING AND SEQUENCING OF BOVINE GRANULOCYTE-COLONY STIMULATING FACTOR CDNA

Author
item HEIDARI, MOHAMMAD - IOWA STATE UNIV., AMES
item Kehrli Jr, Marcus

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/10/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Neutrophils play a critical role in defending against bacterial infections. Hematopoietic growth factors are a class of regulatory cytokines that are required for stimulation, proliferation, and differentiation of blood cells. Granulocyte colony stimulating factor (G-CSF) is a cytokine that induces proliferation and maturation of precursor myeloid cells in the bone marrow into fully differentiated neutrophils. G-CSF also modulates the functional activity of mature neutrophils. Treatment with G-CSF significantly enhances neutrophil phagocytic activity and killing of bacteria and fungi. We have isolated and sequenced a cDNA clone encoding bovine G-CSF from an endothelial cell cDNA library using primers designed from ovine G-CSF. The full length cDNA is 1465 nucleotides long with 522 nucleotides comprising the open reading frame. DNA sequence analysis shows 95% identity with ovine, 89% with porcine, 85% with human, and 77% with murine G-CSF. The deduced G-CSF protein sequence consists of 174 amino acids with 95% identity to ovine, 86% to porcine, 81% to human, and 71% to murine. The signal peptide of G-CSF is 21 amino acids long which is 9 amino acids shorter than that of human and murine. DNA sequence coomparison with an existing bovine G-CSF cDNA in Genbank (Acc. #I09541) reveals a 4 nucleotide difference between the 2 cDNAs. Our sequence difference has been confirmed by sequencing genomic DNA from 5 different breeds of cattle including 4 individual animals from one breed. The possibility of existence of multiple alleles of the gene is under further investigation.