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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Ruminant Diseases and Immunology Research » Research » Publications at this Location » Publication #86609
Title: THE EFFECTS OF BETA-2 INTEGRINS, SOLUBLE PEPTIDES, AND AN OLIGOSACCHARIDE MIMETIC ON NEUTROPHIL ADHERENCE TO EXTRACELLULAR MATRIX PROTEINS

Author
item JONES, SHANNON - IOWA STATE UNIV., AMES
item Kehrli Jr, Marcus
item ACKERMANN, MARK - IOWA STATE UNIV., AMES

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/10/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The degree of adherence of normal bovine neutrophils to keyhole limpet hemocyanin and 3 extracellular matrix proteins (fibronectin, vitronectin, and type I collagen) was determined. These results were compared to those obtained using beta-2 integrin-deficient neutrophils isolated from an animal with bovine leukocyte adhesion deficiency (BLAD). Normal bovine neutrophils bound keyhole limpet hemocyanin (KLH) at a rate 4 times that of beta-2 integrin-deficient neutrophils. There were no substantial differences observed between the normal and beta-2 integrin-deficient neutrophils binding to the extracellular matrix proteins. Additionally, we investigated the effects of several inhibitors of adhesion molecules on neutrophil binding. Activated neutrophils were incubated with RGD (0.1-0.8 mg/ml) to inhibit binding to beta-2 integrins. RGD reduced adherence of normal bovine neutrophils to fibronectin by approximately 40%, yet had negligible effects on adherence of beta-2 integrin-deficient neutrophils. Preliminary evidence suggests that RGD may also inhibit neutrophil binding to collagen, although further investigation is required. RGD had no effect on neutrophil adherence to vitronectin. A selectin blocker, TBC1269, had no significant effect on either normal or beta-2 integrin-deficient neutrophil binding to vitronectin at the concentrations used in this study (0.16-1.28 mg/ml). The results from this study will contribute to the development of a useful clinical therapy for neutrophil-mediated tissue damage.