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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Ruminant Diseases and Immunology Research » Research » Publications at this Location » Publication #86766
Title: EFFECTS OF MASTECTOMY ON THE EXPRESSION OF ADHESION MOLECULES AND FUNCTION OF POLYMORPHONUCLEAR CELLS IN PERIPARTURIENT DAIRY COWS

Author
item KIMURA, KAYOKO - IOWA STATE UNIV., AMES
item Goff, Jesse
item Kehrli Jr, Marcus

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/10/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: We have previously shown the importance of milk production in periparturient immunosuppression by comparing lymphocyte subset populations and function in intact and mastectomized cows. We also hypothesized that milk production affects the polymorphonuclear cells (PMN) as well. To test this hypothesis, we studied the expression of adhesion molecules (CD18 and L-selectin) on PMN by flow cytometry and PMN function by PMN iodination (IOD) using 9 mastectomized and 8 intact multiparous Jersey cows (all intact cows developed milk fever) during the periparturient period. Constitutive and platelet activating factor (PAF) stimulated CD18 expression, exhibited an acute increase at parturition, and fell below preparturient levels from week 1 to week 4 after calving in intact cows. CD18 expression of mastectomized cows did not change during the sampling period. Constitutive and PAF-stimulated expression of L-selectin decreased suddenly at parturition and recovered rapidly after parturition in both groups. PMN-IOD declined gradually before calving (approx. 20 d to 0 d) in both groups and recovered quickly in mastectomized cows, but not in intact cows 20 d after calving. Milk production seems to play an important role in periparturient immunosuppression of PMN especially in terms of recovery from the immunosuppression seen at calving.