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Title: ESTIMATION OF GENOMIC COVERAGE AND GENETIC LENGTH OF THE BOVINE GENOME

Author
item Kappes, Steven - Steve
item LOPEZ-CORRALES, NESTOR - ROSLIN INST., UK
item Heaton, Michael - Mike
item BEATTIE, CRAIG - UNIV. MINNESOTA

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Proceedings
Publication Acceptance Date: 1/1/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Genetic linkage maps have been developed for several livestock species to identify chromosomal regions that influence traits of economic interest. Identifying the chromosomal location of markers from the ends of the linkage groups will indicate the genomic coverage provided by the linkage map and allow estimation of genetic length of the genome. Primer pairs flanking microsatellites from the ends of bovine linkage groups were used to iteratively screen a cosmid library. Cosmid clones containing microsatellites were subsequently assigned to R-banded chromosomes by fluorescence in situ hybridization. Thirty-five new and 25 published physical assignments flank 86% (2560/2990 cM) of the USDA-MARC bovine linkage map. A comparison of the 60 physical assignments and the linkage map suggests that the USDA-MARC bovine linkage map covers ~96% of the genome and the genetic length is ~3100 centimorgans. This information indicates that the linkage map adequately covers the bovine genome for identifying chromosomal regions affecting traits of interest. The improved integration of the physical and linkage maps will augment the use of human and mouse comparative mapping information.