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ARS Home » Plains Area » Houston, Texas » Children's Nutrition Research Center » Research » Publications at this Location » Publication #87712
Title: EFFECT OF ISOPROTERENOL ON THE EXPRESSION OF BETA-ADRENERGIC RECEPTORS IN PIG ADIPOCYTES

Author
item DING, SHIH-TORNG - BAYLOR COLL OF MEDICINE
item MCNEEL, RONALD - BAYLOR COLL OF MEDICINE
item Mersmann, Harry

Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary: An interpretive summary is not required for this abstract.

Technical Abstract: Beta-adrenergic receptors (BetaAR) mediate control of mammalian adipocyte lipid metabolism by BetaAR agonists. To investigate the regulation of BetaAR expression by its agonists, adipocytes were isolated from crossbred pigs weighing 17.0 kg (SD=6.0). Cells were incubated with or without isoproternol, a potent agonist for all BetaAR subtypes. Adipocyte membrane BetaAR binding sites were measured using 125I-cyanopindolol. The transcript levels of Beta1AR and Beta2AR were determined by RNase protection assay using porcine RNA probes and standards. Cells incubated for 6 h, without and with 10 muM isoproternol, had receptor numbers of 347 and 166 fmol/mg protein (P<0.05), respectively. Transcripts for both Beta1AR and Beta2AR were increased by the 10 muM isoproternol treatment. The Beta1AR mRNA increased from 3.39 to 5.74 amol/mug RNA (P=0.07) and the Beta2AR mRNA increased from 0.96 to 1.84 amol/mug RNA (P<0.01). The degradation rates of Beta1AR and Beta2AR mRNAs were not increased by isoproternol treatment. The results suggest that the regulation of Beta1AR and Beta2AR mRNA in pig adipocytes is different from that in rodent adipocytes, in which BetaAR agonists increased the degradation of Beta1AR and Beta2AR mRNAs. Our results demonstrate that the expression of mRNA and receptor protein of BetaAR may be regulated differentially by BetaAR agonists in pig adipocytes.