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ARS Home » Plains Area » Houston, Texas » Children's Nutrition Research Center » Research » Publications at this Location » Publication #87717
Title: CONCENTRATION OF BETA-ADRENERGIC RECEPTOR SUBTYPE TRANSCRIPTS IN PORCINE TISSUES

Author
item MCNEEL, RONALD - BAYLOR COLL OF MEDICINE
item Mersmann, Harry

Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 7/29/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary: An interpretive summary is not required for this abstract.

Technical Abstract: The beta-adrenergic receptor (betaAR) subtypes are differentially distributed in various tissues, yielding divergent pharmacological responses. Ligand binding does not effectively delineate the subtypes in porcine adipose tissue. Consequently, we used ribonuclease protection assays (RPA) to quantify the mRNA for betaAR subtypes in porcine tissues. Segments of the porcine beta1AR, beta2AR, and beta-actin genes were isolated by RT-PCR, cloned, sequenced, and used to produce standards and probes for RPA. After hybridization and RNase digestion, the protected fragments were trapped on glass filters with detection by liquid scintillation counting. The beta1AR and beta2AR transcript concentrations (mean, SD) for 3 to 9 pigs are respectively indicated as amol RNA/mug total RNA: heart = 6.1,1.5 and 2.4, 0.4; lung = 3.8,1.5 and 1.9,0.7; liver = 1.7,0.2 and 2.1,0.9; skeletal muscle = 1.7,0.3 and 1.1,0.4; subcutaneous adipose tissue = 1.3,0.4 and 0.4,0.1. Normalization of data to beta-actin transcript concentration did not change these relationships. The heart, adipose tissue and, surprisingly, the lung had greater beta1AR and beta2AR, whereas in liver and muscle, the subtypes were nearly equal. The quantities of betaAR subtype mRNA provide a baseline for studies of changes in subtypes, and will allow interpretation of tissue pharmacology when coupled with the pharmacology of cloned subtypes.