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Title: MOLECULAR TAGGING OF TOMATO COLOR PIGMENT GENES WITH PCR-BASED MARKERS

Author
item Zhang, Yiping
item Stommel, John

Submitted to: BARC Poster Day
Publication Type: Abstract Only
Publication Acceptance Date: 2/1/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: PCR based molecular markers including RAPD and AFLP were used to identify marker linkage to the genes B (Beta), hp (high pigment), og-c (crimson), and t (tangerine) which are involved in the production of carotenoids in tomato. A total of 1018 RAPD primers and 64 AFLP primer combinations were screened for polymorphism between each of three pairs of near isogenic lines (NILs) which were isogenic for B, hp, and t. Two bulked DNA samples segregating for the locus from an interspecific F-2 population derived from the cross of Lycopersicon cheesmanii accession LA 317 x L. esculentum cv. Floradade were similarly screened. A number of RAPD and AFLP markers detected polymorphism between the two bulks and the paired NILs. Linkages between markers and target genes were confirmed using F-2 and BC populations. Similarly, a single AFLP marker was identified in the B NILs which was linked to the B gene. Linkage with B was confirmed via analysis of a segregating F-2 population from the cross B iso-Rutgers x Rutgers. Additional markers which detected polymorhphism between the respective bulks or pairs of NILs are being evaluated for potential linkage with the hp, og-c, or t phenotype.