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ARS Home » Research » Publications at this Location » Publication #89944
Title: GENOME PAINTING TO DIFFERENTIATE CHROMOSOMES FROM DIFFERENT SOLANUM SPECIES

Author
item DONG, FENGGAO - UNIVERSITY OF WISCONSIN
item Helgeson, John
item JIANG, JIMING - UNIVERSITY OF WISCONSIN

Submitted to: Potato Association of America Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/27/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Introgression of alien chromosomes or chromosomal segments containing useful gene(s) into cultivated potato through wide hybridization or somatic fusion is a valuable method in potato breeding. Effective monitoring of alien chromatin during introgression can increase the efficiency for successful alien gene transfer. By optimizing the genomic in situ hybridization (GISH) technique, we were able to paint chromosomes from S. tuberosum / S. berthaultii and S. etuberosum into different colors, and thus readily identify S. etuberosum chromatin in the S. tuberosum x S. berthaultii + S. etuberosum somatic hybrids and backcross progenies. Genome painting also provided a tool to analyze chromosome pairing between S. tuberosum or S. berthaultii and S. etuberosum. Together with the chromosome-specific DNA markers currently under development, genome painting will allow us to trace individual chromosomes from different Solanum species.