MARKER ASSISTED BREEDING FOR LATE BLIGHT RESISTANCE IN POTATO

Authors: NAESS, S - UNIVERSITY OF WISCONSIN; Haberlach, Geraldine; WIELGUS, SUSAN - UNIVERSITY OF WISCONSIN; RAASCH, JOHN - UNIVERSITY OF WISCONSIN; Helgeson, John

Submitted to: Potato Association of America Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/28/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Late blight has reemerged as a devastating disease on potatoes following the migration of fungicide resistant Phytophthora infestans genotypes into potato producing regions of the U.S.. Resistance to late blight exists in the Mexican diploid, Solanum bulbocastanum. The resistance from this species is currently being introgressed into potato breeding lines following somatic hybridization between the two species. To accelerate the rate of introgression, molecular markers linked to resistance are being sought. Randomly Amplified Polymorphic DNA (RAPD) based mapping of S. bulbocastanum specific markers in 3 BC2 populations segregating for resistance to late blight led to the discovery of a RAPD marker, GO2625, which is tightly linked to resistance. Linkage between resistance and this marker has been confirmed in a BC3 population. Restriction Fragment Length Polymorphism (RFLP) analysis of selected individuals recombinant for the linkage group containing GO2625 indicates that resistance in S. bulbocastanum is located on chromosome 8 and is linked to RFLP marker CT88. Generation of Sequence Characterized Amplified Region (SCAR) markers flanking resistance is in progress. These will allow for the rapid detection of recombinational events in flanking regions which will be useful in further determining the location of the late blight resistance gene/s.