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Title: PCR EVALUATION OF FOURTEEN BEAN GOLDEN MOSAIC VIRUS (BGMV) RESISTANT SNAP BEAN GERMPLASM LINES FOR THE PRESENCE OF THE VIRUS

Author
item MCMILLAN, R - UNIV. FL, TREC/HOMESTEAD
item DAVIS, M - UNIV. FL, TREC/HOMESTEAD
item MCLAUGHLIN, H - UNIV. FL, TREC/HOMESTEAD
item Stavely, J

Submitted to: Bean Improvement Cooperative Annual Report
Publication Type: Other
Publication Acceptance Date: 6/4/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary: Bean Golden Mosaic Virus (BGMV), a DNA plant virus, has been causing increasingly severe losses to commercial snap bean production in south Florida since 1993. Winter and spring production there of this major vegetable for shipment to northern markets is in danger of elimination. Pole snap bean lines homozygous for a pair of genes, designated bgm-1, and thus true breeding for resistance to the virus, have been developed and released by the Agricultural Research Service, USDA with the Florida and Puerto Rico Agricultural Experiment Stations. These resistant plants are asymptomatic, but it has not been known if the virus infects and is present in the resistant plants. Therefore DNA was extracted from asymptomatic plants of 14 resistant lines, including the three released, growing in a field of a highly BGMV susceptible and severely affected cultivar. Analysis for presence of BGMV revealed that 13 of the 14 asymptomatic, resistant plants contained the virus. Thus the virus does invade and multiply in plants with this resistance, suggesting that incorporation of additional resistance may be advisable to ensure stable, durable resistance. Development of BGMV resistant edible bean cultivars will save an important agricultural industry, guarantee a steady winter supply of fresh snap beans for consumers, and provide the public with a fresh, inexpensive major food commodity.

Technical Abstract: Bean Golden Mosaic Virus (BGMV) has recently become the major problem for snap bean production in south Florida, causing millions of dollars of losses. Germplasm lines resistant to BGMV have been developed and released. This resistance is controlled by the recently identified bgm-1 recessive gene. To determine if the virus is present in resistant plants, DNA was extracted from 14 of the resistant lines and analyzed by polymeras chain reaction primers that permit detection of this virus after amplification. The DNA of BGMV was detected in 13 of the 14 sampled lines, indicating that the virus is present in plants having resistance controlled by bgm-1.