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ARS Home » Research » Publications at this Location » Publication #91863
Title: MONILIFORMIN SCREENING IN CORN BY TLC METHOD USING ITS FLUORESCENCE DERIVATIVE WITH 4,5-DICHLORO-1,2-PHENYLENEDIAMINE

Author
item Vesonder, Ronald
item WU, WEIDONG - UNIV OF WI, MADISON
item McAlpin, Cesaria

Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 11/12/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Corn used in starter mash for poultry was examined for fungi and mycotoxins, moniliformin (MON), fumonisins and beauvericin, because the poults exhibited poor growth. Mycological examination of the corn showed it contained predominantly Fusarium proliferatum. Isolates FP1 and FP produced MON when fermented on rice at 25 C in amounts of 240 and 700 ppm, respectively. Each sample of corn and starter mash was extracted with 80% aqueous acetonitrile and each extract applied to a weak anion solid phase cleanup column. The column eluate was screened for MON using a TLC method based on MON ability to form a fluorescence derivative with the reagent 4,5-dichloro-1,2-phenylenediamine. [This reagent was found to form a stronger yellow fluorescence derivative under long UV light than O-phenylenediamine as reported by Dr. George Rottinghaus at the NC129 Meeting on Fusarium Mycotoxins in Cereal Grains (1997)]. Those testing positive for MON were examined by HPLC with UV detection. Moniliformin was detected at 3 ppm in the corn. Low levels of fumonisin (less than 1 ppm was detected and no beauvericin was detected in the corn sample used in the starter mash. Detection limits of MON was about 60 ng per spiked feed sample. This TLC method for MON will be discussed. The starter mash was found to contain about 0.4 ppm of MON. This level of MON would probably not account for the symptoms observed in the poultry.