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Title: SEPARATION AND CHARACTERIZATION OF THE PHOSPHOLIPIDS FROM GLYPHOSATE TOLERANT SOYBEANS

Author
item LIST, GARY
item ORTHOEFER, FRANK - MONSANTO, ST. LOUIS, MO
item TAYLOR, SCOTT
item ABIDI, SHARON
item NELSEN, TERRY

Submitted to: Annual Meeting and Expo of the American Oil Chemists' Society
Publication Type: Abstract Only
Publication Acceptance Date: 5/10/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Phospholipids from three control soybean lines and from two lines of glyphosate tolerant soybeans were isolated from hexane extracted crude oils by column chromatography and further characterized by high pressure liquid chromatography (HPLC). In addition, several lots of commercial fluid lecithin were analyzed and compared to values published in the literature. Phosphatidyl choline (PC), phosphatidyl ethanolamine (PE), phosphatidyl inositol (PI), and phosphatidic acid (PA) were identified as major components in both the experimental cultivars and the commercial lecithin samples. The results showed that glyphosate tolerant beans yielded lecithin comparable to and equivalent to conventional cultivars and the compositions agreed with published literature values. Statistical treatment of the analytical data from duplicate extractions indicated that the largest source of errors were associated with the extraction of the crude oil from the flakes rather than the HPLC separations of the individual phospholipid components.