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ARS Home » Pacific West Area » Aberdeen, Idaho » Small Grains and Potato Germplasm Research » Research » Publications at this Location » Publication #92752
Title: AMPLIFIED DNA FRAGMENT LENGTH POLYMORPHISM BETWEEN OAT AND BARLEY MAPPING PARENTS.

Author
item Hoffman, David
item McNeil, Jill

Submitted to: Oat Newsletter
Publication Type: Other
Publication Acceptance Date: 6/17/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Genetic maps have become an important tool for understanding the basic genetics of crop plants. Detailed molecular maps for oat and barley have been constructed with the use of DNA restriction fragment length polymorphism (RFLP) markers. The use of amplified DNA fragment length polymorphism (AFLP) markers is an efficient method for mapping additional populations and adding more markers to existing maps. Before mapping with AFLP markers, it is essential to know which primer pairs yield numerous DNA amplification differences between parental cultivars. The objectives of this study were to 1) identify which AFLP primer pairs will yield the most DNA amplification differences among oat mapping parents and 2) compare this information with that found for barley. Total genomic DNA was extracted from plants of oat mapping cultivars Kanota, Ogle, and TAM O-301, and from plants of barley mapping cultivars Steptoe and Morex. AFLP was conducted using protocols established by Gibco BRL Life Technologies, and the five cultivars were surveyed for potential AFLP markers with 62 EcoRI-MseI primer pairs. The results indicated that some primer pairs are better than others in producing AFLPs in both oat and barley, and that the conduct of a preliminary screen is helpful for efficient mapping. AFLP was more readily found in barley. Some adjustments to the procedure may need to be made to find more AFLP for oat. The next step will be to determine the actual number of AFLP markers with segregating populations. This information will be used to help construct an oat molecular genetic map in Ogle/TAM O-301 and increase the number of non-RFLP markers for barley.