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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #93420
Title: RFLP MARKER ASSOCIATION TO CHROMOSOME USING 'SUN II' OAT ANEUPLOIDS

Author
item START, MARYANN - UNIVERSITY OF MINNESOTA
item Rines, Howard
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA

Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 10/18/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The molecular genetic linkage map for cultivated hexaploid oat (2n=6x=42) now consists of 33 linkage groups. The objective of this research is to reduce the number of linkage groups to 21, the expected number for hexaploid oat, by assigning linkage groups to physical chromosomes using a nearly complete Sun II monosomic series. A series of 20 oat monosomics (absent mono 3C) has been assembled, with each missing a different chromosome based on C-banding analysis. Fourteen of the monosomics were newly derived from Sun II oat haploids generated in oat by maize crosses. Three of the monosomics were derived by Hacker and Riley in a different selection of Sun II and have a 3C-14 interchange. Three monosomics recovered in the cultivar 'Kanota', as described by Morikawa, have been backcrossed with Sun II. Monosomic F1s from crosses of the monosomic lines with Kanota and 'Ogle' were identified by mitotic chromosome counts of root ttips, and leaf tissue was collected for DNA extraction. RFLP autoradiograms will be analyzed and missing bands will allow assignment of sequences to the monosomic chromosomes.