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Title: QTL MAPPING OF OAT QUALITY TRAITS: ACETYL-COA CARBOXYLASE, A CANDIDATE GENE FOR OIL CONTENT

Author
item KIANIAN, SHAHRYAR - NORTH DAKOTA STATE UNIV
item GROH, SUSANNE - UNIVERSITY OF MINNESOTA
item EGLI, MARGARET - UNIVERSITY OF MINNESOTA
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA
item Rines, Howard
item SOMERS, DAVID - UNIVERSITY OF MINNESOTA
item GENGENBACH, BURLE - UNIVERSITY OF MINNESOTA
item WEBSTER, FRANCIS - THE QUAKER OATS COMPANY
item LIVINGSTON, SUZANNE - UNIVERSITY OF MINNESOTA
item O'DONOUGHUE, LOUISE - DNA LANDMAKRS INC, CANADA

Submitted to: American Oat Workers Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/27/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Genetic manipulation of groat quality traits such as oil and beta-glucan content is desired for optimizing the value of oats in human and livestock diets. The localization and characterization of quantitative trait loci (QTLs) for these traits is necessary for identifying genetic markers to be used for marker-assisted selection (MAS) to improve the quality of oat groats. Two populations of recombinant inbred lines derived from the crosses Kanota x Ogle (KxO) and Kanota x Marion (KxM) were genotyped using RFLP markers and tested for oil and beta-glucan content across several locations and years. QTL analysis was conducted using composite interval mapping with 350 RFLP loci in KxO and single factor ANOVA with 60 loci in KxM. A major QTL affecting oil content was found consistently across environments in both populations on linkage group 11, explaining up to 43% of the phenotypic variance. A second locus was found in each population, explaining an additional 10 to 20% of the phenotypic variance. A cDNA clone encoding oat acetyl-CoA carboxylase (ACCase) mapped to the same region as the major QTL on linkage group 11 in both populations. ACCase, which catalyzes the first step in de novo fatty acid synthesis, is therefore likely to play a major role in determining groat oil content and provides an excellent marker for MAS. Furthermore, the genomic region around an ACCase gene locus also showed an effect on beta-glucan, indicating that selection for this locus could simultaneously influence oil and beta-glucan content. Additional research will be needed to gain a better understanding of the role of ACCase in determining groat oil content and possible effects on correlated traits.