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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #93572
Title: OAT PLANTS WITH INTROGRESSED MAIZE CHROMOSOME SEGMENTS FROM GAMMA IRRADIATION OF PROGENY OF OAT X MAIZE CROSSES

Author
item RIERA-LIZARAZU, OSCAR - UNIVERSITY OF MINNESOTA
item VALES, ISABEL - UNIVERSITY OF MINNESOTA
item ANANIEV, EVGUENI - UNIVERSITY OF MINNESOTA
item Rines, Howard
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA

Submitted to: American Oat Workers Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/27/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Crosses of oat (Avena sativa L., 2n = 6x = 42) by maize (Zea mays L. 2n = 2x = 20) have resulted in the recovery of both haploid oat plants (2n = 21) and plants with 21 oat chromosomes plus one or more retained maize chromosomes. From these, self-fertile disomic addition lines (2n = 42 + 2) with a full complement of 21 oat chromosome pairs plus an added maize chromosome pair were recovered for 6 of the 10 maize chromosomes. The phenotypes of these oat-maize chromosome addition lines ranged from that of near normal oat plants to quite marked effects on vigor and plant morphology. The added maize chromosome represents a potential source of new traits for oat such as disease resistance or seed composition modification. In addition, for maize genomics analyses, the addition lines represent a valuable source for mapping or isolating maize DNA sequences specific to a particular maize chromosome. Irradiation of these whole chromosome addition nlines to induce the transfer of maize chromosome segments into the oat genome was seen as a way to further reduce the amount and complexity of maize chromatin present. We treated oat maize chromosome 9 monosomic seed with gamma rays (30, 40, and 50 k rad). Progeny plants from self- fertilization of plants from irradiated seed were screened for the presence of maize DNA by Southern analysis using a multiprobe of a mixture of maize- specific repeated sequences. The maize chromatin present was characterized by fluorescence genomic in situ hybridization of root tip cells and by analyzing DNA extractions with a series of 30 maize RFLP and SSR markers specific for chromosome 9. Various maize chromosome 9 rearrangements in oat were identified including translocation introgressions of maize chromosome segments onto oat chromosomes.