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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #93574
Title: CHARACTERIZATION OF A AND C GENOME-SPECIFIC DISPERSED AND CLUSTERED REPETITIVE DNA SEQUENCES FROM AVENA SATIVA

Author
item ANANIEV, EVGUENI - UNIVERSITY OF MINNESOTA
item VALES, ISABEL - UNIVERSITY OF MINNESOTA
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA
item Rines, Howard

Submitted to: American Oat Workers Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/27/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The genomic DNAs from eight species of oat with A, C, AB, AC, and ACD genomes were used as probes in Southern blot hybridization to a blot panel of cosmid clones from A. sativa (ACD) genomic DNA cut with different restriction enzymes. Several DNA subfragments were identified which specifically hybridized with labeled A or C genomic DNA. Those subfragments swere recloned and characterized. Among them, eight A/D-genome and five C- genome specific DNA sequences have been identified as members of different families of dispersed repetitive elements. In situ hybridization of an A/D genome-specific repeat, termed As-A1, to metaphase chromosomes of A. sativa showed that it is dispersed along 14 out of 21 A. sativa chromosome pairs, representing the A and D genomes. One of the C genome-specific repeats, termed As-C1, is dispersed along 7 of the 21 pairs of A. sativa chromosomes. Six of the seven C genome chromosome pairs have chromosomal segments of different length at their termini which gave hybridization wit the As-A1 but not with the As-C1 repeats while termini of six out of fourteen AD genome chromosome pairs gave hybridization with the As-C1 but not with the As-C1 repeats. Thus, these two probes, As-A1 and As-C1, enabled the detection of presumably ancient reciprocal terminal translocation segments of the C chromosomes and A or D chromosomes. A second C genome-specific repeat, termed As-C2, revealed clusters on six of the seven C chromosome pairs. These three genome-specific repetitive DNA sequences were used to screen the A. sativa genomic cosmid library to selectively isolate C or A/D genome-specific cosmid clones. Analysis of these new clones revealed that they contain additional genome- or chromosome-specific DNA probes.