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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #93576
Title: AN UPDATE OF THE MOLECULAR LINKAGE MAP OF CULTIVATED OAT

Author
item GROH, SUSANNE - UNIVERSITY OF MINNESOTA
item TINKER, NICK - AGRIC & AGRI-FOOD CANADA
item O'DONOUGHUE, LOUISE - DNA LANDMARKS INC, CANADA
item KIANIAN, SHAHRYAR - NORTH DAKOTA STATE UNIV
item FOX, STEPHEN - UNIV OF SASKATCHEWAN
item LIVINGSTON, SUZANNE - UNIVERSITY OF MINNESOTA
item WIGHT, CHARLENE - AGRIC & AGRI-FOOD CANADA
item Rines, Howard
item SORRELLS, MARK - CORNELL UNIVERSITY
item MOLNAR, STEVE - AGRIC & AGRI-FOOD CANADA

Submitted to: American Oat Workers Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/27/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: A molecular genetic linkage map of cultivated oat based on the cross Kanota (Avena byzantina) x Ogle (A. sativa) has been developed by a consortium effort. This map has provided the foundation for additional genetic studies and is a pivotal component of ongoing comparative-mapping efforts. We have continued to update this map since it was first reported by O'Donoughue et al. (Genome 38:368-380, 1995). Here, we report an updated version of the Kanota x Ogle linkage map. The former map was based on 561 loci mapped in 71 recombinant inbred lines (RILs). We have now mapped additional RFLP markers detected by cDNA clones from oat, barley, and wheat as well as probes from oat genomic DNA. After checking for duplicates and severe segregation distortion, data were available for 703 marker loci. The population has been extended to 137 RILs, in which 108 of the loci have been mapped to date. Markers were assigned to linkage groups at LOD 7. Some unlinked groups have been joined based on chromosome assignments determined by aneuploid studies (reported elsewhere), resulting in a total of 32 linkage groups. Marker ordering within linkage groups was performed for a subset of 259 loci providing a robust framework with a uniform marker density of approximately 5 to 10 cM. Most ordering within this framework was performed at LOD 3. Markers not included in the framework have been placed relative to this framework. The current size of this base map is 1770 cM excluding unlinked markers. Future work will include mapping of additional markers on the extended population, with emphasis on markers that will allow for comparisons with maps of other crop species. Updated versions of the map will be made available on the GrainGenes database (http://wheat.pw.usda.gov/).