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Submitted to: Proceeding of World Aquaculture Society
Publication Type: Abstract Only Publication Acceptance Date: 1/27/1999 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Ichthyophthirius multifiliis is an obligate parasite and upkeep of laboratory stocks requires serial infestation, thus, a regular supply of fish. A reliable, low maintenance method of maintaining an I. multifiliis culture is needed in our labs in order to use this common parasite as a model for research in therapeutics evaluation for the approval of new drugs by the U.S. Food and Drug Administration. With this in mind, our research has been focused on cryopreservation of this ciliated protozoan. Few attempts have been made to cryopreserve I. multifiliis and the results of these studies have not been encouraging. The initial task of the present research was to determine the toxicity of several cryopreservants to I. multifiliis theronts. The toxicity of diethylene glycol, N, N dimethyl acetamide, dimethyl sulfoxide, glycerol, methanol, and sucrose was observed at various concentrations and at 15 min intervals for 1 h. Toxicity of the cryoprotectant was influenced by the developmental stage of the theronts; observations were done at 12 and 24 h after harvesting of trophonts from the fish. A computer operated controlled-rate freezer was used in attempts to cryopreserve I. multifiliis theronts and the |
