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Title: PHENOTYPE REVERSION IN RELATION TO AGROBACTERIUM TUMEFACIENS MEDIATED TRANSFORMATION OF NON-RIPENING TOMATO MUTANT

Author
item BARTOSZEWSKI, GRZEGORZ - WARSAW AGRIC UNIV POLAND
item FEDOROWICZ, OLGA - WARSAW AGRIC UNIV POLAND
item MALEPSZY, STEFAN - WARSAW AGRIC UNIV POLAND
item Smigocki, Anna
item NIEMIROWICZ, KATARZYNA - WARSAW AGRIC UNIV POLAND

Submitted to: Polish Genetics Society Conference
Publication Type: Abstract Only
Publication Acceptance Date: 9/22/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The most characteristic feature of the non-ripening tomato nor mutant is the yellow colour of fruits at the stage of full maturity. These fruits do not synthesise climacteric ethylene or accumulate lycopene. The nor gene has been subject to intensive studies due to its impact on ethylene production. In the recent experiments the nor mutant was transformed with three Agrobacterium tumefaciens strains which contained different binary plasmids (pBI121, pRUR528 and pHSCKn312). In total, 18 transgenic plants were generated (5 for pBI121, 5 for pRUR528 and 8 for pHSCKn312) and three of them showed normally ripening red fruits. All the other plants as well as the plants regenerated in vitro as a control for the transformation experiments had yellow fruits, typical of the nor mutant. These results suggest the influence of transformation events on phenotype reversion. Futher research is needed to check the nor gene stability and to identify the reasons of reversion.