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ARS Home » Research » Publications at this Location » Publication #94925
Title: DNA FINGERPRINTING OF BORDETELLA AVIUM AND BORDETELLA HINZII ISOLATES

Author
item Sacco, Randy
item Nordholm, Gwen

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/28/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Bordetella avium causes respiratory tract infections in poultry (coryza or rhinotracheitis). B. hinzii is the name recently given to organisms previously referred to as B. avium-like or Alcaligenes faecalis type II. Although these latter organisms have been isolated from diseased birds, there is little evidence that they are pathogenic. Four isolations of B. hinzii from humans have been reported, including isolations from blood of an AIDS patient and sputum of a patient with cystic fibrosis. The objective of the experiments reported herein was to determine if DNA fingerprinting could be utilized to distinguish among B. avium and B. hinzii strains representing isolates from the United States, South Africa, and Germany. Digestion of chromosomal DNA with twenty restriction enzymes was carried out for DNA fingerprint analysis. The digested DNA fragments were electrophoresed in agarose gels and stained with ethidium bromide. We found that restriction enzyme digestion with Hinf I produced fingerprin patterns in the 2.0- to 10-kb range, and readily distinguished B. avium isolates from B. hinzii isolates. Furthermore, Hinf I digestion of chromosomal DNA from B. avium strains produced eight distinct fingerprint profiles.