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Title: RESPONSES TO NITROGEN-STIMULATION AND TO IMMUNIZATION WITH AN UNRELATED ANTIGEN OF CATTLE NATURALLY INFECTED WITH GASTROINTESTIONAL NEMATODES AFTER TRANSPORT TO A FEEDLOT

Author
item Gasbarre, Louis
item GOMEZ-MUNOZ, T - UNIV COMPLUTENSE, SPAIN
item ALMERA, S - INIA, SPAIN
item Canals, Ana
item GUERRERO, J - MERIAL INC NJ
item ROBERTSON, C - MERIAL INC NJ

Submitted to: American Association of Veterinary Parasitologists Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/24/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Angus-based stocker cattle were kept on parasite infected pastures for 150 days in Fulton MO. Cattle were divided into 3 treatment groups, each of which received a different anthelmintic treatment ranging from no treatment to the administration of an IVOMEC R Sustained Release Bolus with efficacy for the entire grazing period. Approximately one month before the end of the grazing one-half of each treatment group (30 animals per treatment) were immunized with 5 mg of keyhole limpet hemocyanin (KLH) without adjuvant. The remaining one-half of the animals were given a similar immunization after all animals were sent to a feed lot at Canyon TX one month later. Two weeks after the appropriate immunization, peripheral blood mononuclear cells were collected from the immunized cattle for the assessment of cellular immune responses to KLH and to 2 different concentrations of the mitogen CON A. After an additional 2 weeks, immunized cattle were bled once more for the measurement of anti-KLH 1gG1 and 1gG2 antibodies and serum pepsinogen levels. Serum pepsinogen determinations indicated significant differences among all 3 treatment groups at both times tested, with the group receiving no anthelmintic displaying the highest levels of serum pepsinogen. After shipment to the feedlot, all groups showed greatly reduced antibody responses after KLH immunization when compared to the responses of all groups before transport. In addition, the group receiving no anthelmintic had significantly lower reponses after CON A stimulation when compared to both anthelmintic treated groups. Differences in grazing cattle before transport were less clear because of sigificant effects of both the pasture and pasture by treatment interaction.