Skip to main content
ARS Home » Research » Publications at this Location » Publication #96261
Title: MONOCLONAL ANTIBODIES SPECIFIC FOR E. COLI OF SEROTYPES 026 AND 0111

Author
item RIVERA BETANCOURT, MILDRED
item KEEN, JAMES

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/1/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: E. coli O26 and O111 are classic enteropathogenic (EPEC) serotypes which have been linked with diarrheagenic illness in both humans and animals. More recently, E. coli O26 and O111 producing Shiga-like toxins have emerged as the most important non-O157 enterohemorrhagic E. coli (EHEC) and have been associated with both sporadic cases and large outbreaks of severe ehuman disease such as hemorrhagic colitis and hemolytic-uremic syndrome. In order to facilitate the identification and detection of EPEC and EHEC O26 and O111, two monoclonal antibodies (MAbs 12F5-C7 and 15C4-D5) of the IgM and IgG3 isotypes, respectively, were generated from splencocytes of BALB/c mice immunized with E. coli O26:H11 and O111:NM whole cell lysate. The MAbs' sensitivity and specificity were evaluated by indirect ELISA reactivity with a total of 247 bacterial isolates. These included 212 E. coli (28 O26, 23 O111, and 161 non-O26/non-O111) of various pathotypes and O:H serotypes as well as 35 non-E. coli (9 Salmonella spp and 26 other Gram-negative bacteria). MAb 12F5-C5 reacted specifically with all 28 E. coli O26 strains. MAb 15C4-D5 reacted with all 23 E. coli O111. The anti-O26 MAb cross-reacted with 1 of 219 non-E. coli O26, while the anti-O111 MAb cross-reacted with 2 of 224 non-E. coli O111. The anti-E. coli O111 MAb also reacted with 8 of 8 Salmonella O35. This was expected since the Salmonella O35 and E. coli O111 O-antigens are structurally identical. The high specificities of these two MAbs may permit their use as immunoreagents for detection of enteropathogenic and enterohemorrhagic E. coli O26 and O111 in livestock or food products or as serotyping reagents.