Author
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Wesley, Irene |
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Franklin, Sharon |
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SCHROEDER-TUCKER, L - USDA, APHIS, NVSL |
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MURANO, E - TEXAS A&M UNIVERSITY |
Submitted to: Compendium of Methods for the Microbiological Examination of Foods
Publication Type: Book / Chapter Publication Acceptance Date: 8/16/1999 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Microbes of the genus Arcobacter are aerotolerant Campylobacter-like organisms which grow at 25-30 C. Arcobacter butzleri has been recovered from healthy livestock, poultry products, and from cases of human enteritis. The characteristics of Arcobacter, its distribution, epidemiology, and limited information regarding its pathology are reviewed. The methods for isolation and identification of these potential human foodborne pathogens are described. Especial emphasis is placed on enrichment in EMJH-P-80 semi-solid media followed by confirmation via the polymerase chain reaction. This protocol has been developed by USDA and has been extensively field tested. Methods for fingerprinting isolates of A. butzleri, including restriction fragment length polymorphism and PCR-based DNA fingerprinting are also detailed. |